INTEGRATIVE MOLECULAR BIOLOGY LAB BIO366

Description

The Integrative Molecular Biology Lab research group has a multidisciplinary profile with lines focused on different biological problems: the study of cancer, nitrogen and sulphur metabolisms in plants or the study of methionine modifications as a regulator of protein activity. The group carries out biotechnology work using tools from biochemistry, molecular biology, and bioinformatics. Among the techniques handled by the research group we can highlight molecular cloning of genes, recombinant protein expression, enzyme activity measurements, protein phosphorylation studies, gene expression analysis (RT-qPCR), protein expression analysis (western blot) and omics analysis (e.g. transcriptomics).

Research Topics

Protein evolution and post-translational modifications.
Nitrogen and sulphur nutrition in plants.
Cell signalling and cancer.

Scientific-Technical Services

Technical advice for the realization of Biotechnological projects.
Molecular cloning including recombinant protein expression and characterization.
Gene expression analysis by conventional (RT-qPCR) and high throughput (RNA-seq and/or DRS) techniques.
Culture and maintenance of cell lines.
Functional assays in tumor cell lines.
Gene editing by siRNA or CRISPR-Cas9.
Proliferation and/or toxicity assays.
Cell signaling pathway analysis.
Immunodetection.